Recombinant Rat CD16a/FCGR3A Protein (His Tag)(Active)

Name: Recombinant Rat CD16a/FCGR3A Protein (His Tag)(Active)
SKU: EWV8411672988
Price: 98.99 USD
Availability: InStock

$98.99 ~~$1.00~~

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Category : Life Science Products

Recombinant Rat CD16a/FCGR3A Protein (His Tag)(Active)

SKU #	PKSR030403
Expression Host	HEK293 Cells

Description

Synonyms	Fcgr4
Species	Rat
Expression Host	HEK293 Cells
Sequence	Met 1-Pro 200
Accession	XP_ 008767961.1
Calculated Molecular Weight	21.7 kDa
Observed Molecular Weight	28-33 kDa
Tag	C-His
Bio-activity	Immobilized rat FCGR3A-His at 10 g/ml (100 l/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 59.0-139.0 ng/ml.

Properties

Purity	> 97 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per g of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.