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AccuRapid Protein Synthesis Kit allows the expression and purification of desired proteinsin vitrowithout culturing cells.This product contains all the components required forE.colicell-free expression and Ni-affinity purification experiments, and can recover up to 100 g of purified target protein per reaction.
This product is shipped in dry ice and ice pack by cartridge.
Convenience
Simple synthesis of protein without the need of cells, also allowing expression of proteins that can't be done by conventional cell culture methods.
User-friendly
Easy identification of the expression and purification of your desired protein from a variety of templates (plasmids or PCR products) that can be expressed in the T7 system.
Compatibility
Compatible with the world's first fully automatic protein synthesis equipment, ExiProgen EC Protein Synthesis Kit.
Box No. | Components | Size | Storage Temperature |
1 | Ni-NTA magnetic bead | 50 mg x 5 ea | 4 |
Elution buffer | 1.25 ml x 1 ea | ||
Binding/washing buffer | 25 ml x 1 ea | ||
2 | E. coliextract | 200 l 5 ea | -70 |
Master mix | 350 l 5 ea | -20 | |
Positive control DNA | 10 l x 1 ea | ||
DEPC D.W. | 1 ml 1 ea |
E. coliextract:As T7 RNA polymerase and ribosome are included, it is recommended to be stored at temperatures below -70 immediately upon the receive. Performance will decrease upon repeated freezing and thawing.